Method and device for isoelectric focusing without carrier...

Details Method and device for isoelectric focusing without carrier... Method and device for isoelectric focusing without carrier...

G - Physics

01

N

G01N 27/447 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2157373

A device and method for isoelectric focusing of ampholytes in a buffer uses a cone-shaped capillary with a positive electrode connected at a narrow end and a negative electrode connected at the wide end of the capillary. The electrical potential across the buffer creates a temperature gradient which, in turn, creates a pH gradient The electric current also creates an electric field gradient which focuses the ampholytes. Previous devices and methods use carrier ampholytes or use temperature baths to create a temperature gradient.

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