Method and device for measuring the concentration of a...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/58 (2006.01) C12M 1/34 (2006.01) C12M 1/40 (2006.01) C12Q 1/34 (2006.01) G01N 27/02 (2006.01) G01N 27/06 (2006.01)

Patent

CA 2196161

Methods and apparatus for measuring the concentration of decomposable substances, such as urea, are disclosed. The disclosed methods include adding a gaseous buffer, such as CO2, to the solution containing the decomposable compound, measuring the conductivity of the solution, decomposing the decomposable compound, measuring the conductivity of the thus-decomposed compound solution, and calculating the differential conductivities between the two measured solutions. The apparatus for carrying out these methods are also disclosed.

Méthode et dispositif de mesure de la concentration de l'urée ou d'une substance similaire, dans une solution composite, notamment une solution de dialyse, par décomposition catalytique de l'urée par l'uréase. On mesure la différence de conductivité entre la solution ayant subi la réaction et la solution ne l'ayant pas subi, cette conductivité étant proportionnelle à la concentration d'urée. La décomposition s'opère selon la réaction suivante: (NH2)2 CO + CO2 + 3H2O } 2NH4<+> + 2HCO3<-> (5). Sous l'effet de l'addition (41, 60) de dioxide de carbone sous forme gazeuse et de la catalysation par l'uréase, la réaction se déplace vers la droite, ce qui signifie que cette proportionalité s'applique sur une vaste plage de concentrations d'urée, jusqu'à et même au-delà de 35 mM. L'addition de dioxide de carbone sous forme gazeuse assure également une valeur de pH suffisamment basse dans la solution pour prévenir la précipitation de carbonate de calcium.

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