Method and device for obtaining a sample with...

G - Physics – 02 – B

Patent

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G02B 21/06 (2006.01) G01N 21/64 (2006.01) G02B 21/22 (2006.01) G03H 1/08 (2006.01)

Patent

CA 2451166

The invention concerns a method for obtaining a sample with three-dimensional microscopy, in particular a thick biological sample, and the fluorescence field emitted by said sample. The invention is characterised in that it comprises: a step of measuring and recording, which consists in measuring and recording sequentially and independently the interferometric signals obtained by digital holography and the fluorescence signals, derived from the sample; a processing step which consists in combining said interferometric signals obtained by digital holography and said thus recorded fluorescence signals so as to reconstruct clear three-dimensional images of the sample itself and of the fluorescence field emitted by the sample at a given time.

La présente invention se rapporte à un procédé pour obtenir par microscopie des images en trois dimensions d'un échantillon, en particulier d'un échantillon biologique épais, et du champ de fluorescence émis par cet échantillon, caractérisé en ce qu'il comprend:- une étape de mesure et enregistrement, au cours de laquelle on mesure et on enregistre de façon séquentielle et indépendante d'une part des signaux interférométriques obtenus par holographie digitale et d'autre part des signaux de fluorescence, issus de l'échantillon ;- une étape de traitement, au cours de laquelle on combine lesdits signaux interférométriques obtenus par holographie digitale et lesdits signaux de fluorescence ainsi enregistrés, de manière ô reconstruire des images nettes, en trois dimensions, de l'échantillon lui-même et du champ de fluorescence émis par ledit échantillon à un instant donné.

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