Method and device for prepaping polynucleotide microarray...

G - Physics – 01 – N

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G01N 33/53 (2006.01) B01J 19/00 (2006.01) G01N 33/52 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2408767

A method of constructing a polynucleotide microarray whereby a sufficient amount of polynucleotide can be immobilized on a support and thus a uniform and strong signal can be obtained in a spot area even in the case of synthetic oligopolynucleotides which are known as being hardly immobilized via electrostatic bonds because of short chain length. Namely, a method of constructing a polynucleotide microarray characterized in that a solution in which a polynucleotide is dissolved in the step of immobilizing the polynucleotide on a support comprises water or a mixture of water with an organic solvent and is substantially free from any anionic electrolytes, and, in case where the solution comprises a mixture of water with an organic solvent, the content of the organic solvent in the solution is less than 50% by volume.

L'invention concerne un procédé de construction d'un microréseau de polynucléotides, selon lequel une quantité suffisante de polynucléotides peut être immobilisée sur un support, et ainsi un signal fort et uniforme obtenu dans une zone, même dans le cas d'oligopolynucléotides synthétiques connus pour être difficiles à immobiliser au moyen de liens électrostatiques à cause de leur courte longueur de chaîne. Plus spécifiquement, l'invention concerne un procédé de construction d'un microréseau de polynucléotides, caractérisé en ce qu'une solution, dans laquelle le polynucléotide est dissous à l'étape d'immobilisation du polynucléotide sur un support, contient de l'eau ou un mélange d'eau avec un solvant organique, et ne contient essentiellement aucun électrolyte anionique. En outre, dans le cas où cette solution comprend un mélange d'eau avec un solvant organique, la teneur en solvant organique dans la solution représente moins de 50 % en volume.

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