C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
150/15, 150/26
C12M 1/34 (2006.01) C12Q 1/00 (2006.01) G01N 30/90 (2006.01) G01N 33/558 (2006.01)
Patent
CA 2009111
METHOD AND DEVICE FOR QUANTITATIVE CHROMATOGRAPHY ABSTRACT OF THE DISCLOSURE An improved chromatographic device and method for quantitating the activity of an enzyme utilizes a bibulous test strip as the stationary phase, an aqueous solution as the mobile phase, and a chromophore as the indicator. The enzyme activity is quantitated by transforming a substantially depletable amount of a chromogenic substrate from soluble chromogen to insoluble chromophore as the mobile phase advances, thereupon the insoluble chromophore becomes immobilized on the test strip to produce a column of color. The length and/or the intensity of the column of color are related to the activity of the enzyme. The present invention is useful for the direct determination of enzyme-analytes and for the indirect determination of analytes which can be coupled to an enzyme.
Abbott Laboratories
Goudreau Gage Dubuc
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