Method and device for screening molecules in cells

G - Physics – 01 – N

Patent

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Details

G01N 33/50 (2006.01) B01J 19/00 (2006.01) B01L 3/00 (2006.01) G01N 1/28 (2006.01) G01N 35/10 (2006.01)

Patent

CA 2492933

The invention concerns a method for reacting a reagent R with a cell C, which consists in: setting the cell C on a support S comprising a substantially planar surface, in the form of an aqueous drop on said surface; covering the planar surface of the support S whereon the aqueous drop has been set containing the cell C with a separation film F, allowing through gases and preventing the aqueous drops set on the support S from evaporating, F being non-miscible with the reagent R; triggering the reaction between the reagent R and the cell C by introducing the reagent R in the aqueous drop containing the cell C. The invention also concerns a device for implementing said procedure and its applications.

L'invention concerne un procédé de mise en réaction d'un réactif R avec une cellule C, dans lequel :- la cellule C est déposée sur un support S comportant une surface sensiblement plane, sous forme d'une goutte aqueuse sur ladite surface,- la surface plane du support S sur laquelle a été déposée la goutte aqueuse contenant la cellule C est recouverte par un film de séparation F, permettant le passage des gaz et empêchant l'évaporation des gouttes aqueuses déposées sur le support S, F étant non miscible avec le réactif R,- la réaction entre le réactif R et la cellule C est déclenchée par l'introduction du réactif R dans la goutte aqueuse contenant la cellule C.L'invention concerne également un dispositif pour la mise en uvre de ce procédé et ses utilisations.

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