Method and device for separating molecular targets in a...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) B01J 19/00 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2598508

The invention relates to a method of analysing molecular targets contained in a complex mixture, comprising the following steps consisting in: a) bringing the mixture of molecular targets to be analysed into contact with an array of different types of primary probes, whereby each type of primary probe forming the array can bind specifically to a type of target selected from among the molecular targets, under conditions that enable specific binding between the molecular targets and the primary probes; b) optionally eliminating the primary probes that are not bound specifically to a molecular target; c) separating the molecular targets and the primary probes which are bound specifically in a probe/target complex, such as to recover the array of primary probes representing a fingerprint of the molecular targets to be analysed; and d) quantitatively analysing the primary probes eluted in step c.

L'invention a pour objet un procédé d'analyse de cibles moléculaires contenues dans un mélange complexe comprenant : a) la mise en contact du mélange de cibles moléculaires à analyser avec un jeu de sondes primaires de types différents, chaque type de sonde primaire composant le jeu étant susceptible de se lier par liaison spécifique à un type de cible parmi les cibles moléculaires, dans des conditions permettant la liaison spécifique entre lesdites cibles moléculaires et lesdites sondes primaires, b) éventuellement l'élimination des sondes primaires non spécifiquement liées avec une cible moléculaire, c) la séparation des cibles moléculaires et des sondes primaires liées par liaison spécifique dans un complexe sonde-cible, de façon à récupérer le jeu de sondes primaires représentant une empreinte des cibles moléculaires à analyser, d) l'analyse quantitative des sondes primaires éluées à l'étape c.

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