Method and device for separation and depletion of certain...

Details Method and device for separation and depletion of certain... Method and device for separation and depletion of certain...

G - Physics

01

N

G01N 27/447 (2006.01)

Patent

CA 2656991

The present invention provides a novel and advantageous method for separating analytes by free flow electrophoresis. The methods are particularly suitable for depleting major constituents such as albumin from samples of biological origin, optionally combined with a further separation of the remaining portion of the sample. The sample portions recovered from the method can be used advantageously in downstream applications such as 1D or 2D-PAGE, HPLC or mass spectrometric analysis. Also provided are buffer systems, kits comprising such buffer systems, and devices for carrying out the free flow electrophoretic separation methods of the present invention.

La présente invention concerne un nouveau procédé avantageux destiné à séparer des analytes par une électrophorèse libre. Les procédés sont particulièrement adaptés pour appauvrir les constituants principaux tels que l'albumine à partir d'échantillons d'origine biologique, facultativement en combinaison avec une séparation supplémentaire de la partie restante de l'échantillon. Les parties d'échantillon récupérées à partir du procédé peuvent être utilisées avantageusement dans des applications aval telles que l'électrophorèse 1D ou 2D-PAGE, une HPLC ou une analyse par spectrométrie de masse. L'invention concerne également des systèmes tampons, des trousses comprenant de tels systèmes tampons et des dispositifs destinés à réaliser les procédés de séparation par électrophorèse libre de la présente invention.

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