Method and device for specific binding assay

Details Method and device for specific binding assay Method and device for specific binding assay

G - Physics

01

N

G01N 33/543 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/53 (2006.01) G01N 33/558 (2006.01) G01N 33/74 (2006.01)

Patent

CA 2152756

This invention provides a specific binding assay method which is excellent in general purpose applicability and can perform highly accurate and quick measurement effected by the exclusion of various factors that decrease reliability of the measured values, such as non-specific reactants in test samples, assay conditions and inactivation and the like changes in the activity of reagents, as well as a specific binding assay device suitable for the practice thereof. This object is achieved by allowing a signal substance generator which takes part in a specific binding reaction and generates a signal substance, together with a liquid sample, to flow through a predetermined channel in a predetermined direction, thereby effecting generation of the specific binding reaction of a substance to be assayed to form a distribution of the signal substance generator in the channel in response to the concentration of the substance to be assayed, allowing the signal substance generator distributed in the channel to generate the signal substance, detecting the generated signal substance by a plurality of detection means arranged at different positions in the liquid flow direction, and arithmetically processing the plural detection results to minimize influence of other factors than the concentration of the substance to be assayed upon the assay result.

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