Method and device for specifically detecting myoglobin using...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/28 (2006.01) C12M 1/40 (2006.01) G01N 33/72 (2006.01)

Patent

CA 2168625

A method and device for detecting myoglobin amongst other mammalian peroxidatively active substances like hemoglobin is disclosed. Since the existing peroxidase-sensitive components do not discriminate between myoglobin and hemoglobin, the present method and device provide discrimination by including a filtration step or component which separates myoglobin from hemoglobin on the basis of their difference in molecular weights. The present method and device offer a quick and easy detection of myoglobin, especially in urine, and may conveniently use existing reagents and apparatus.

Le présent brevet porte sur une méthode et un dispositif pour détecter la myoglobine parmi d'autres substances sensibles à la peroxydase chez les mammifères comme l'hémoglobine. Étant donné que les composants sensibles à la peroxydase qui existent ne font pas la différence entre la myoglobine et l'hémoglobine, la méthode et le dispositif présentés permettent de faire la différence en incluant une filtration ou un composant qui sépare la myoglobine de l'hémoglobine selon le poids moléculaire. La méthode et le dispositif présentés constituent une méthode de détection rapide et facile de la myoglobine, en particulier dans l'urine, et peuvent utiliser sans inconvénient les réactifs et les appareils existants.

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