C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/08 (2006.01) A61K 35/14 (2006.01) B01D 17/02 (2006.01) B01D 21/24 (2006.01) B01D 21/26 (2006.01) B04B 5/04 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 1/34 (2006.01) G01N 1/40 (2006.01) G01N 33/49 (2006.01) G01N 33/569 (2006.01)
Patent
CA 2154748
A method and apparatus for creating a leukocyte rich sample from a mixed population of blood cells. The method comprises the steps of: separating an amount of blood into layers that include a buffy coat layer having a mixed population of blood cells including plasma and red blood cells; providing a chamber having an opening for receiving a fluid; causing the buffy coat layer to enter the chamber; centrifuging the chamber to separate the buffy coat layer into at least a plasma containing layer, a leukocyte containing layer, and a red blood cell containing layer; and separating the leukocyte containing layer from the other layers in the chamber.
L'invention concerne une méthode et un appareil pour produire un échantillon riche en leucocytes à partir d'une population mixte de cellules sanguines. La méthode consiste à séparer une certaine quantité de sang en couches comprenant une couche leucocytaire contenant une population mixte de cellules sanguines renfermant du plasma et des globules rouges; à prévoir une chambre ayant une ouverture pour recevoir un fluide; à introduire la couche leucocytaire dans la chambre; à centrifuger la chambre pour séparer la couche leucocytaire en au moins une couche contenant le plasma, une couche contenant les leucocytes et une couche contenant les globules rouges; et à séparer la couche contenant des leucocytes des autres couches dans la chambre.
Bratten William
Chapman John
Martinson Jeffrey
Wang Li Ming
Baxter International Inc.
Sim & Mcburney
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