C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
C12M 3/02 (2006.01) C12M 1/00 (2006.01) C12M 3/04 (2006.01)
Patent
CA 2458941
The invention relates to a method for the in vitro cultivation of cells, which grow on a culture surface. According to the invention, the cells are sown on culture surfaces (1) and are cultivated in a culture medium, whereby the culture surface is continually or periodically expanded, without being removed from the culture medium. To expand the culture surface, the cells are not detached from the culture surface and the culture surface between the cells is expanded. However, at least part of the cells can be detached and the culture surface can be expanded by the flooding of additional culture surface areas. The culture surface of an example device for said cell cultivation consists of one side of an expanding membrane (6), which is expanded by modifying the pressure on the other side. Said cell cultivation can be carried out without manual passaging. The cells are subjected to less stress than in known cell cultivation methods and said method can be automated more easily.
L'invention concerne un procédé de culture in vitro de cellules se développant sur une surface de culture. Ce procédé comprend les étapes consistant à : semer les cellules sur des surfaces de culture (1) et à les cultiver dans un milieu de culture ; agrandir la surface de culture continuellement ou périodiquement sans la retirer du milieu de culture. Pour agrandir la surface de culture, les cellules ne sont pas séparées de la surface de culture et celle-ci est agrandie entre les cellules. Néanmoins, au moins une partie des cellules peuvent être séparées de la surface de culture et cette dernière peut être agrandie par inondation de zones de surface de culture supplémentaires. La surface de culture d'un dispositif exemplaire de culture cellulaire consiste en une face d'une membrane expansible (6) agrandie par modification de la pression appliquée sur l'autre face. La culture cellulaire peut s'effectuer sans passage manuel. Le procédé de culture cellulaire selon l'invention soumet les cellules à moins de contraintes que les procédés de culture cellulaire connus et peut plus facilement être automatisé.
Frei Heribert
Mainil-Varlet Pierre
Mueller Werner
Arbomedics Gmbh
Freedman Gordon
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