Method and device for treating or selecting cells

B - Operations – Transporting – 01 – L

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Details

B01L 3/00 (2006.01) C12M 3/06 (2006.01) C12N 5/08 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2648144

The present invention is directed to a method of treating cells by co-culturing them with activated fibroblasts in order to regulate the growth and/or status of the cells. Fibroblasts are activated by culturing the cells under conditions that induce the cells to adhere to each other to form multicellular aggregates or spheroids. The present invention also provides a device for selecting cells from cell samples, such as bone marrow aspirate, the device comprises said multicellular aggregates.

La présente invention concerne un procédé de traitement de cellules consistant à les cultiver conjointement à des fibroblastes activés en vue d'une régulation de la croissance et/ou du statut desdites cellules. Les fibroblastes sont activés en cultivant les cellules dans des conditions qui induisent l'adhérence des cellules les unes aux autres, de façon à former des agrégats ou des sphérules multicellulaires. La présente invention concerne également un dispositif destiné à la sélection de cellules à partir d'échantillons cellulaires, tels qu'un prélèvement de moelle osseuse, le dispositif comprenant lesdits agrégats multicellulaires.

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