C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) G01N 33/543 (2006.01)
Patent
CA 2239499
The invention concerns a method and device for treatment by complexing of a liquid medium containing one or several constituents determining the presence in said liquid medium of a plurality of ligands, which consists in: a) providing a support for plural complexing, on which are distributed a plurality of coupling sites, in which are immobilised a plurality of anti-ligands respectively different; b) contacting said liquid medium with said complexing support, whereby said ligands are paired with said anti-ligands. The invention is characterised in that the stability of each ligand/anti-ligand complex being dependent of at least one exogenous parameter, called reference parameter, conditioning said support, the coupling sites on the support are differentiated, depending on said exogenous reference parameter, having respectively different values in said sites, and the anti-ligands are distributed among the different coupling sites, depending on the predetermined optimum values of the exogenous reference parameter, determining the stability of the different ligand/anti-ligand complexes respectively. The invention is useful for sequencing nucleic acids by hybridisation.
Procédé et dispositif de traitement par complexation d'un milieu liquide comprenant un ou plusieurs composants déterminant la présence dans ledit milieu liquide d'une pluralité de ligands, selon lequel: a) on dispose d'un support de complexation plurale, sur lequel sont distribués une pluralité de sites de couplage, dans lesquels sont immobilisés une pluralité d'anti-ligands respectivement différents, b) on met en contact ledit milieu liquide avec ledit support de complexation, moyennant quoi lesdits ligands s'apparient avec lesdits anti-ligands, caractérisé en ce que la stabilité de chaque complexe ligand/anti-ligand étant dépendante, d'au moins un paramètre exogène, dit de référence, conditionnant ledit support, d'une part on différencie les sites de couplage sur le support, en fonction dudit paramètre exogène de référence, ayant des valeurs respectivement différentes dans lesdits sites, et d'autre part on distribue les anti-ligands entre les différents sites de couplage, en fonction de valeurs optimum prédéterminées du paramètre exogène de référence, déterminant la stabilité des différents complexes ligand/anti-ligand respectivement. Application au séquençage d'acides nucléiques par hybridation.
Bio Merieux
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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