Method and device for withdrawing a volume of liquid by...

G - Physics – 01 – N

Patent

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Details

G01N 30/18 (2006.01) B01L 99/00 (2010.01) B01L 3/00 (2006.01) G01N 30/16 (2006.01) G01N 30/20 (2006.01) G01N 35/10 (2006.01)

Patent

CA 2642293

The invention relates to a method for withdrawing a volume of liquid by suction, in particular for collecting a sample for analysis by means of a liquid chromatography device, wherein the liquid comprising the volume of liquid to be withdrawn by suction is provided in a container (7, 75) closed by means of a closure element (72, 71; 76, 761) which, at least in a partial area, is made of a flexible material that can be pierced by means of a sampling needle, wherein, in order to withdraw a defined volume of liquid by suction, the sampling needle (6) is pushed through the partial area made of flexible material, until the suction opening of the sampling needle reaches sufficiently deep into the liquid, and wherein a defined volume of liquid is withdrawn by suction. According to the invention, in order to avoid a situation where the withdrawal of the volume of liquid causes an underpressure to develop in the container (7, 75), a relative movement between the sampling needle (6) and the container (7, 75) in a direction substantially perpendicular to the longitudinal axis of the sampling needle (6) has the effect that the opening (9) generated in the flexible material of the closure element (72, 71; 76, 761), by the flexible material being pierced, is widened in such a way that, between the outer wall of the sampling needle (6) and the inner wall of the widened opening (9), a complete or partial equalization of pressure is permitted between the container volume and the environment.

L'invention concerne un procédé d'aspiration d'un volume de liquide, notamment de prélèvement d'un échantillon aux fins d'analyse à l'aide d'un dispositif de chromatographie en phase liquide. Le liquide contenant le volume de liquide à aspirer se trouve dans un contenant (7, 75) fermé par un couvercle (72, 71; 76, 761) qui est constitué, au moins dans une zone partielle, d'un matériau flexible apte à être transpercé par une aiguille de prélèvement. Pour procéder à l'aspiration d'un volume de liquide défini, on pique l'aiguille de prélèvement (6) dans la zone partielle de matériau flexible jusqu'à ce que l'orifice d'aspiration de l'aiguille de prélèvement soit immergé à une profondeur suffisante dans le liquide, un volume de liquide défini étant aspiré. L'invention vise à éviter que le prélèvement du volume de liquide ne génère un vide partiel dans le contenant (7, 75). A cet effet, un mouvement relatif est réalisé entre l'aiguille de prélèvement (6) et le contenant (7, 75) dans une direction sensiblement perpendiculaire à l'axe longitudinal de l'aiguille de prélèvement (6) de telle façon que l'orifice (9) réalisé par piquage dans le matériau flexible du couvercle (72, 71; 76, 761) soit agrandi afin de permettre, entre la paroi externe de l'aiguille de prélèvement (6) et la paroi interne de l'orifice (9), une compensation de pression complète ou partielle entre le volume du contenant et l'environnement.

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