G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/543 (2006.01) G01N 33/53 (2006.01) G01N 37/00 (2006.01)
Patent
CA 2475240
A method of determining an amount of analyte in a sample solution is provided. The method involves the use of an assay device that has a substantially planar assay surface. The surface has a plurality of calibration dots a test dot printed thereon. The calibration dots contain pre-determined quantities of the analyte while the test dot includes a capture antibody for binding to the analyte. The analyte is mixed into a solution having a sample antibody for the analyte, where the antibody is labeled with a detectable marker. The sample solution is introduced into the loading portion of the assay device for delivery to said reading portion. The next step is to measure the intensity of detectable marker in the calibration dots. With the data obtained, one then prepares a calibration curve correlating the amount of analyte in said calibration dots to said intensity of detectable marker. The intensity of detectable marker in the test dot can be measures and the amount of anayte present in said test dot calculated by comparing the intensity of detectable marker to the amount of analyte corresponding to the intensity in said calibration curve.
de Leo Domenica
Lea Peter
Liu Jun
Biophys Inc.
Dimock Stratton Llp
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