G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/543 (2006.01) G01N 33/53 (2006.01) G01N 33/545 (2006.01) G01N 37/00 (2006.01)
Patent
CA 2475456
The present invention provides a method of making an assay device for conducting an assay to detect a concentration of an analyte protein in a sample fluid. The assay devices would typically have a substantially planar surface having a plurality of calibration spots containing pre-determined quantities of the analyte protein printed thereon. In addition, a test dot including a capture antibody for binding the analyte protein is printed on the assay device. The method involves first modifying the planar surface to provide hydrophobic binding sites and hydrophilic linking sites. Then the method requires printing the test spots and the calibration dots on the substantially planar surface. Applying the sample fluid to the assay device is the next step followed by testing a sensitivity of the assay and altering a ratio of the hydrophobic binding sites to the hydrophilic linking sites in order to optimize the sensitivity of the assay.
Biophys Inc.
Dimock Stratton Llp
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