Method and devices for detecting and enumerating microorganisms

Details Method and devices for detecting and enumerating microorganisms Method and devices for detecting and enumerating microorganisms

C - Chemistry, Metallurgy

12

Q

C12Q 1/02 (2006.01)

Patent

CA 2297140

A method for detecting a microorganism in a test sample is described. The method involves distributing microvolumes 0.01-25 microlitres of a sample to a plurality of microcompartments of a culture device, incubating for a time sufficient to permit at least one cell division cycle of the microorganism, then detecting the presence or absence of the microorganism in the microcompartments. Also disclosed are devices for carrying out these methods.

Cette invention se rapporte à un procédé de détection d'un micro-organisme dans un échantillon pour essai. Ledit procédé consiste à répartir des microvolumes (0,01 à 25 microlitres) d'un échantillon dans une pluralité de microcompartiments d'un dispositif de culture, à laisser incuber pendant un laps de temps suffisant pour permettre au moins un cycle de division cellulaire du micro-organisme, puis à déceler la présence ou l'absence du micro-organisme dans les microcompartiments. L'invention se rapporte également à des dispositifs permettant de mettre en oeuvre ce procédé.

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