Method and devices for partitioning biological sample...

Details Method and devices for partitioning biological sample... Method and devices for partitioning biological sample...

C - Chemistry, Metallurgy

12

M

C12M 1/20 (2006.01) B01L 3/00 (2006.01) C12Q 1/04 (2006.01)

Patent

CA 2286195

A method for partitioning an aqueous biological liquid sample into discrete microvolumes for detection and enumeration of microorganisms is described. The method involves distributing microvolumes of a sample to a plurality of hydrophilic liquid-retaining zones of a culture device, where each liquid-retaining zone is surrounded by a portion of a hydrophobic "land" area. Also disclosed are devices for carrying out these methods.

Cette invention se rapporte à un procédé permettant de diviser un échantillon aqueux de liquide biologique en microvolumes discrets en vue de la détection et du dénombrement de micro-organismes. Ledit procédé consiste à répartir les microvolumes d'un échantillon dans une pluralité de zones hydrophiles de rétention de liquides d'un dispositif de culture, chacune de ces zones de rétention de liquides étant entourée par une partie de surface de "terrain" hydrophobe. L'invention se rapporte également à des dispositifs conçus pour la mise en oeuvre d'un tel procédé.

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