Method and formulation for stabilization of enzymes

C - Chemistry – Metallurgy – 12 – N

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C12N 9/96 (2006.01) C12N 9/12 (2006.01) C12N 9/16 (2006.01) C12N 9/22 (2006.01)

Patent

CA 2259138

The present invention is directed to a method and a formulation for the stabilization of an enzyme for storage at temperatures above freezing. Specifically, the invention involves the use of formulated excipients that when added to an enzyme or mixture of enzymes and subsequently lyophilized renders stability. Upon reconstitution, the stabilized materials are useful in assays, diagnostic or molecular biology research kits, and other biological applications.

La présente invention concerne un procédé et une formulation permettant la stabilisation d'un enzyme en vue de sa conservation à des températures supérieures à la température de congélation. L'invention concerne en particulier l'utilisation d'excipients formulés qui, lorsqu'ils sont ajoutés à un enzyme ou à un mélange d'enzymes et sont par la suite lyophilisés, ont un pouvoir stabilisant. Après reconstitution, les matières stabilisées sont utiles pour dosages, diagnostic ou kits de recherche en biologie moléculaire, ainsi que pour d'autres applications biologiques.

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