Method and kit for demonstrating microorganisms

Details Method and kit for demonstrating microorganisms Method and kit for demonstrating microorganisms

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) G01N 33/569 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2095843

2095843 9208805 PCTABS00013 Detection of microorganisms by first isolating them from the sample by means of antibodies and a solid support for the antibodies and then, optionally after isolation of the DNA from the bound microorganisms, carrying out a PCR. The antibodies to be used are specific for the microorganisms to be detected and most preferably generated against still living microorganisms. Especially magnetic particles are suitable as solid support. In PCR, primers are used that are specific for the microorganisms to be detected, most preferably based on an rRNA sequence or a DNA replication origin sequence.

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