Method and kit for detecting membrane protein-protein...

Details Method and kit for detecting membrane protein-protein... Method and kit for detecting membrane protein-protein...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12Q 1/37 (2006.01) G01N 33/50 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2479999

The present invention is concerned with a method and a kit for detecting an interaction between a first membrane bound test protein or fragment thereof and a second test protein or fragment thereof which is either membrane bound or soluble with an in vivo genetic system based in yeast, bacterial or mammalian cells. The system makes use of the reconstitution of the split ubiquitin protein.

La présente invention concerne un procédé et un kit pour détecter une interaction entre une première protéine test liée par membrane ou son fragment et une deuxième protéine test ou son fragment, liée ou soluble dans un système génétique <i>in vivo</i> basé dans la levure, les cellules bactériennes ou mammaliennes. Le système utilise la reconstitution de la protéine d'ubiquitine fendue.

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