Method and kit for determining tyrosine-phosphorylating and...

Details Method and kit for determining tyrosine-phosphorylating and... Method and kit for determining tyrosine-phosphorylating and...

G - Physics

01

N

G01N 33/68 (2006.01) C07K 16/18 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2050317

A safe and sensitive method is provided for determining the tyrosine- phosphorylating or phosphotyrosine-dephosphorylat- ing activity of a material, such as a human tissue sample, by reacting the sample with a polypeptide which contains (phospho)tyr- osine residues and assaying the phosphorylated polypeptide by using an antibody to a polypeptide containing phosphotyrosine. the method can conveniently be performed using a diagnosis kit containing the polypeptide and the antibody. The method and the kit are useful for example for diagnosing tumours.

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