Method and kit for immuno-detecting bacteria in blood tissues

G - Physics – 01 – N

Patent

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Details

G01N 33/569 (2006.01) G01N 33/566 (2006.01) G01N 33/58 (2006.01) G01N 33/60 (2006.01)

Patent

CA 2422833

The invention provides methods for screening for the presence of a clinically relevant amount of bacteria in donor blood or a blood product from a donor mammal, particularly blood or a blood product that will be transferred from the donor mammal to a recipient mammal. The method comprises contacting a sample of the donor blood or a blood product with a set of binding agents that comprises binding agents that specifically bind to Gram-negative bacterial antigen and/or binding agents that specifically bind to Gram-positive bacterial antigen, and determining binding of the set of binding agents to the sample, wherein binding indicates the presence of a clinically relevant amount of Gram-positive bacteria and/or Gram- negative bacteria in the donor blood or blood product and no binding indicates the absence of a clinically relevant amount of Gram- positive bacteria and/or Gram-negative bacteria in the donor blood or blood product. The invention further provides methods and kits for screening for the presence of a clinically relevant amount of Gram-positive bacteria, Gram-negative bacteria, or both Gram- positive and Gram-negative bacteria in a donor tissue by screening the fluid in which the donor tissue is stored.

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