Method and kit for isolation and purification of...

Details Method and kit for isolation and purification of... Method and kit for isolation and purification of...

G - Physics

01

N

G01N 33/535 (2006.01) C07K 1/16 (2006.01) C07K 16/00 (2006.01) C12N 9/08 (2006.01) C12N 9/16 (2006.01)

Patent

CA 2157177

A process is described for isolating an enzyme-antibody conjugate, wherein the enzyme is horseradish peroxidase or alka- line phosphatase, from an aqueous mixture of said conjugate and unconjugated enzyme. The process involves contacting the mix- ture with a water insoluble stationary phase having the Ni2+ ion chelated thereto and binding said conjugate to the stationary phase. The phase containing bound conjugate is then washed to remove unbound enzyme. Thereafter the conjugate is eluted from the stationary phase and recovered in a form substantially free of the unconjugated enzyme as shown in the figure.

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