Method and kit for measuring anti-ebna antibodies

Details Method and kit for measuring anti-ebna antibodies Method and kit for measuring anti-ebna antibodies

G - Physics

01

N

G01N 33/569 (2006.01) C07K 14/05 (2006.01) C07K 16/08 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2065602

ABSTRACT OF THE DISCLOSURE This invention provides a simple immunoassay system and a measuring kit for use in the measurement of anti-EBNA antibodies, in which recombinant type EBNA proteins are used. The anti-EBNA antibody measuring kit is useful for the individual measurement of anti-EBNA-1 antibodies, anti-EBNA-2 antibodies, anti-EBNA-3 antibodies, anti-EBNA-4 antibodies, anti-EBNA-5 antibodies and anti-EBNA-6 antibodies. Particularly, this invention provides a method for the measurement of anti-EBNA antibodies which comprises the steps of allowing anti-EBNA antibodies in a test sample to react with a recombinant type EBNA fusion protein immobilized on a support through an anti-.beta.-galactosidase antibody, allowing the anti-EBNA antibodies to react with an enzyme-conjugated anti-EBNA antibody and measuring a signal (enzyme activity) generated from the conjugated enzyme contained in an immune complex formed by the above steps. Also particularly, this invention provides an anti-EBNA antibody measuring kit in which an anti-.beta.-galactosidase antibody and a recombinant type EBNA fusion protein are immobilized on a support through binding of the anti-.beta.-galactosidase antibody to the support and binding of the recombinant type EBNA fusion protein to the anti-.beta.-galactosidase antibody.

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