Method and kit for quantitatively determining small, dense...

Details Method and kit for quantitatively determining small, dense... Method and kit for quantitatively determining small, dense...

C - Chemistry, Metallurgy

12

Q

C12Q 1/26 (2006.01) C12Q 1/30 (2006.01) C12Q 1/42 (2006.01) C12Q 1/44 (2006.01)

Patent

CA 2702172

A method for fractional measurement of small, dense LDL, which is adaptable for an autoanalyzer, and a reagent for measurement, are provided, making it possible to conduct rapid and convenient analysis with good sensitivity without pretreatment of a specimen. The method for quantitatively determining small, dense LDL cholesterol in a sample comprises the steps of: (1) eliminating cholesterol in LDL other than small, dense LDL in the presence of phospholipase; and (2) quantitatively determining cholesterol in lipoproteins remaining in step (1) above.

La présente invention concerne un procédé et un réactif permettant de réaliser la mesure fractionnelle d'une petite particule dense de LDL, en vue d'une analyse rapide, simple et extrêmement spécifique qui ne nécessite pas le prétraitement de l'échantillon. En outre, ladite mesure est applicable à un appareil d'analyse automatique. La présente invention concerne spécifiquement un procédé de quantification de petites particules denses de cholestérol LDL dans un échantillon, qui comprend les étapes (1) et (2) suivantes : (1) désactivation de tout le cholestérol dans les LDL autres que les petites LDL denses en présence d'un phospholipase; et (2) mesure de la quantité d'un cholestérol dans une lipoprotéine qui reste après l'étape (1).

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