C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/00 (2006.01) C07K 16/28 (2006.01)
Patent
CA 2702230
The present invention relates to methods for isolating human forkhead box P3 (Foxp3+) CD4+ regulatory T cells (herein referred to as Foxp3+ Treg cells) from a sample containing (i) peripheral blood mononuclear cells (PBMCs), (ii) a lymphocyte containing fluid, or (iii) a lymphocyte containing tissue, a kit for isolating human Foxp3+ Treg cells, and the use of anti-CD49d antibody for the isolation of human Foxp3+ Treg cells.
La présente invention porte sur des procédés pour isoler des lymphocytes T régulateurs CD4+ de type forkhead box P3 (Foxp3+) humains (appelés présentement cellules Treg Foxp3+) à partir d'un échantillon contenant (i) des cellules mononucléaires de sang périphérique (PBMC), (ii) un fluide contenant des lymphocytes, ou (iii) un tissu contenant des lymphocytes. L'invention porte également sur une trousse pour isoler des cellules Treg Foxp3+ humaines et sur l'utilisation d'anticorps anti-CD49d pour l'isolement de cellules Treg Foxp3+ humaines.
Falk Kirsten
Kleinewietfeld Markus
Roetzschke Olaf
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