Method and kit for the detection of the response of an...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2387878

This invention provides methods or another transcription product and kits for the chemiluminescent detection of mRNA transcribed from genetic material in response to exposure of an organism, cell or genetic assembly to a chemical or physical change in its environment. These exposures result in an alteration in the level of activity of genes or transcription pathways responsive to such changes in such a way that the amount of mRNA associated with this activity changes.

L'invention concerne des méthodes ou un autre produit de transcription et des kits servant à la détection par chimiluminescence d'ARNm transcrit à partir de matériel génétique en réponse à l'exposition d'un organisme, d'une cellule ou d'un ensemble génétique à un changement chimique ou physique dans son environnement. Ces expositions produisent une altération du niveau d'activité des gènes ou des mécanismes de transcription sensibles à ces changements de telle manière que la quantité d'ARNm associée à cette activité s'en trouve modifiée.

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