Method and kit for the determination of antibodies

G - Physics – 01 – N

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G01N 33/564 (2006.01) G01N 33/543 (2006.01) G01N 33/563 (2006.01) G01N 33/577 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2130486

2130486 9415214 PCTABS00032 For the determination of antibodies (Ak), in particular of autoantibodies, such as human autoantibodies against thyroid peroxidase (hTPO), a procedure is adopted in which the antibodies to be determined are bound to a solid or undissolved phase in a conventional manner using unspecific binders and are then selectively labelled, for a subsequent measurement, by reaction with a crude antigen preparation (Ag) and a further, labelled antibody or antibody fragment (F(AK)*) which are specific for the antigen but are not bound, or not significantly bound, by the binder. The presence of the antibodies (AK) to be determined manifests itself in the form of a label bound to the solid or undissolved phase, the amount of said label being proportional to the amount of the antibodies to be determined in the sample.

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