Method and kits for preparing multicomponent nucleic acid...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/66 (2006.01) C07H 21/00 (2006.01) C12N 15/63 (2006.01) C12N 15/64 (2006.01)

Patent

CA 2258570

The invention provides a highly efficient, rapid, and cost effective method of linking nucleic acid components in a predetermined order to produce a nucleic acid multicomponent construct. Such a method and multicomponent construct are illustrated in the figure. The invention further provides nucleic acid components, each nucleic acid component comprising a double stranded nucleic acid molecule having at least one single stranded 5' or 3' terminal sequence, the terminal sequence having sufficient complementarity to either a terminal sequence in a separate nucleic acid component or a sequence in a linking nucleic acid molecule so as to allow for specific annealing of complementary sequences and linkage of the components in a predetermined order. Kits containing reagents required to practice the method of the invention are also provided.

L'invention concerne un procédé hautement efficace, rapide et rentable de liaison de constituants d'acide nucléique dans un ordre prédéterminé afin de produire une construction multiconstituant d'acide nucléique. L'invention concerne également des constituants d'acide nucléique, chaque constituant comprenant une molécule d'acide nucléique bicaténaire présentant au moins une séquence terminale 5' ou 3' monocaténaire, la séquence terminale présentant une complémentarité suffisante vis-à-vis soit d'une séquence terminale dans un constituant d'acide nucléique séparé, soit d'une séquence dans une molécule d'acide nucléique de liaison, afin de permettre un annelage spécifique de séquences complémentaires et la liaison des constituants dans un ordre prédéterminé. L'invention concerne également des kits contenants des réactifs nécessaires à la mise en pratique du procédé de l'invention.

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