Method and materials for producing proteins

Details Method and materials for producing proteins Method and materials for producing proteins

C - Chemistry, Metallurgy

12

N

195/1.22, 195/1.

C12N 15/68 (2006.01) C12N 1/20 (2006.01) C12N 5/10 (2006.01) C12N 15/18 (2006.01)

Patent

CA 2022356

ABSTRACT OF THE DISCLOSURE The invention provides a hybrid mRNA molecule comprising a first RNA sequence coding for a protein or polypeptide of interest operatively linked to a second RNA sequence which is capable of stabilizing the hybrid mRNA in the presence of estrogen and estrogen receptor. The hybrid mRNA molecule may further comprise a third sequence located between the first and second sequences which codes for a cleavage site so that the protein or polypeptide of interest may be cleaved from unnecessary or interfering amino acid sequences coded for by the second RNA sequence. The invention further provides a recombinant DNA sequence coding for the hybrid mRNA, a vector comprising the recombinant DNA sequence operatively linked to expression control sequences, and a host cell transformed with the vector. Finally, the invention provides a method of stabilizing an mRNA so as to obtain increased production of a protein or polypeptide. The method comprises growing the transformed host cell in the presence of estrogen.

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