G - Physics – 06 – K
Patent
G - Physics
06
K
195/1.14, 195/1.
G06K 15/00 (2006.01) B41J 9/00 (2006.01) B41J 3/12 (1985.01)
Patent
CA 1259043
IMPROVED METHOD AND MEANS FOR MICROBIAL POLYPEPTIDE EXPRESSION Abstract of the Disclosure The Specification discloses: 1. Recombinant microbial cloning vehicles com- prising heterologous DNA coding for the expression of mammalian hormone (e.g., somatostatin) and other polypeptides, including plasmids suited for the trans- formation of bacterial hosts. The latter incorporate a regulon homologous to the host in its untransformed state, in reading phase with the structural gene for the heterologous DNA; 2. Cloning vehicles coding for the microbial expression of a protein variously comprising (a) a poly- peptide hapten and additional protein sufficient in size to confer immunogenicity on the product of expression, which may find use in raising antibodies to the hapten for assay use or in the manufacture of vaccines; and (b) a desired polypeptide product and additional protein from which the desired product may be cleaved; and 3. Methods of preparing synthetic structural genes coding for the expression of mammalian polypeptides in microbial cloning systems.
514816
Genentech Inc.
Gowling Lafleur Henderson Llp
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