Method and means for producing high titer, safe, recombinant...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/867 (2006.01) C12N 15/48 (2006.01) C12N 15/63 (2006.01) C12N 15/64 (2006.01)

Patent

CA 2370103

Lentiviral vectors modified at the 5' LTR or both the 5' and 3' LTR are useful in the production of recombinant lentivirus vectors (See the Figure). Such vectors can be produced in the absence of a functional tat gene. Multiple transformation of the host cell with the vector carrying the transgene enhances virus production. The vectors can contain inducible or conditional promoters.

L'invention concerne des vecteurs lentiviraux modifiés au niveau de 5' LTR ou au niveau de 5' et 3' LTR utiles dans la production de vecteurs lentiviraux recombinants (voir fig. 1). Ces vecteurs peuvent être obtenus en l'absence d'un gène fonctionnel tat. Les multiples transformations de la cellule hôte avec le vecteur porteur du transgène contribuent à l'amélioration de la production de virus. Les vecteurs peuvent contenir des promoteurs inductibles ou conditionnels.

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