Method and medium for producing cholesterol oxidase

Details Method and medium for producing cholesterol oxidase Method and medium for producing cholesterol oxidase

C - Chemistry, Metallurgy

12

P

195/123

C12P 21/00 (2006.01) C12N 9/04 (2006.01) C12N 9/18 (2006.01)

Patent

CA 1067844

Abstract of the Disclosure An improved fermentation method comprises growing a cholesterol oxidase-producing microorganism in a medium comprising a carbon source, such as glycerol, yeast extract, a nonionic surfactant, an auxiliary carbon source which is also an inducer of cholesterol oxidase, and trace salts. Yields of cholesterol oxidase up to about 900 U. (international units) per liter have been obtained using the improved method of the present invention.

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