C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/53 (2006.01) C12N 1/14 (2006.01) C12N 1/20 (2006.01) C12N 9/04 (2006.01) C12N 15/63 (2006.01) C12P 7/18 (2006.01) C12P 19/02 (2006.01)
Patent
CA 2501391
The invention relates to a method and an organism for the production of D- mannitol. The production of D-mannitol can be improved by providing said method and microorganism, using an organism expressing mannitol-2- dehydrogenase (MDH) and formate-dehydrogenase (FDH, for co-factor regeneration). The sugar substrates and/or sugar substrate precursors of MDH are transported via a non-phosphorylating sugar transport system into the organism. The sugar transport system is, advantageously, a glucose facilitator (GLF) from Zymomonas mobilis.
L'invention concerne un procédé et un organisme servant à produire du D-mannitol au moyen d'un organisme exprimant une mannitol 2 déshydrogénase (MDH) et une formiate déshydrogénase (FDH, pour la régénération de cofacteur), les substrats sacchariques ou les précurseurs de substrats sacchariques de la MDH étant transportés dans l'organisme par l'intermédiaire d'un système de transport de sucres non phosphorylant. Le procédé et l'organisme selon l'invention permettent d'améliorer la production de D-mannitol. On utilise de manière avantageuse comme système de transport de sucres le facilitateur de glucose (GLF) provenant de Zymomonas mobilis.
Bringer-Meyer Stephanie
Hemmerling Claudia
Kaup Bjoern
Sahm Hermann
Walter Martin
Forschungszentrum Juelich Gmbh
Nordzucker Innocenter Gmbh
Ridout & Maybee Llp
LandOfFree
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