F - Mech Eng,Light,Heat,Weapons – 25 – D
Patent
F - Mech Eng,Light,Heat,Weapons
25
D
F25D 17/02 (2006.01) A01N 1/02 (2006.01) B65D 55/06 (2006.01) B65D 77/20 (2006.01) B65D 85/50 (2006.01)
Patent
CA 2210532
This invention is directed to an efficient cryopreservation package desgin of harvested mammalian tissues and living cultured tissue equivalents made by in vitro technology. The invention involves immersing a mammalian tissue or cultured tissue equivalent in a cryoprotectant solution, agitating the cryoprotectant solution and the immersed tissue to achieve effective penetration of the cryoprotectant solution into the tissue, and then freezing the tissue at a very slow freezing rate. In the freezing step, extracellular ice formation is initiated by seeding. The cryopreserved tissue may be stored for indefinite periods of time prior to use. The cultured tissue equivalent is an in vitro model of the equivalent human tissue, such as skin or cornea, which, when retrieved from storage can be used for transplantation or implantation in vivo or for screening compounds in vitro.
L'invention concerne une nouvelle structure d'emballage de cryoconservation efficace de tissus mammaliens récoltés et d'équivalents de tissus de culture obtenus par la technique in vitro. Le procédé selon l'invention consiste à immerger un tissu mammalien ou un équivalent de tissu de culture dans une solution cryoprotectrice, à agiter la solution cryoprotectrice et le tissu immergé de sorte que la solution cryoprotectrice pénètre efficacement dans le tissu, et à congeler le tissu à une vitesse de congélation très faible. Dans la phase de congélation, la formation de glace extracellulaire est induite par ensemencement. Le tissu cryoconservé peut être stocké pendant des périodes indéfinies avant d'être utilisé. L'équivalent de tissu de culture est un modèle in vitro du tissu humain équivalent tel que la peau ou la cornée, qui, une fois retiré du stockage peut être utilisé pour une transplantation ou un implant in vivo ou pour le titrage de composés in vitro.
Toner Mehmet
Tschumakow Alexander G.
Watson Stephen R.
Kirby Eades Gale Baker
Organogenesis Inc.
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