Method and polynucleotides for determining translational...

Details Method and polynucleotides for determining translational... Method and polynucleotides for determining translational...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2357804

A method is disclosed for determining the translational efficiency of an individual codon in a cell. The method comprises introducing into the cell a synthetic construct comprising a reporter polynucleotide fused in frame with a tandem repeat of said individual codon, wherein said reporter polynucleotide encodes a reporter protein, and wherein said synthetic construct is operably linked to a regulatory polynucleotide and measuring expression of said reporter protein in said cell to determine the translational efficiency of said codon.

La présente invention concerne un procédé permettant de déterminer l'efficacité de la traduction d'un codon individuel dans une cellule. Ce procédé consiste à introduire dans la cellule une construction synthétique comprenant un polynucléotide marqueur fusionné dans un cadre avec une séquence nucléotidique répétée en tandem dudit codon individuel. Le polynucléotide marqueur code une protéine marqueur et ladite construction synthétique est liée fonctionnellement à un polynucléotide régulateur. Le procédé consiste ensuite à mesurer l'expression de ladite protéine marqueur dans la cellule afin de déterminer l'efficacité de la traduction du codon.

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