Method and probes for the detection of a tumor specific...

G - Physics – 01 – N

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G01N 33/542 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2505509

This invention relates to the detection of fusion proteins. The invention provides a set of at least a first and a second molecular probe, each probe provided with a dye wherein said dyes together allow energy transfer; at least one probe provided with a reactive group allowing juxtaposing said at least first and second probe wherein said reactive group allows to modulate juxtaposing said probes such that there is an increased likelihood of energy transfer between said dyes. A method is provided which permits detecting the presence of a fusion protein in a cell at the single cell level.

L'invention se rapporte à la détection de protéines de fusion et à un ensemble d'au moins une première et une seconde sondes moléculaires, chacune présentant un colorant permettant le transfert d'énergie une fois les colorants assemblés ; au moins une sonde pourvue d'un groupe réactif permettant la juxtaposition d'au moins une des deux sondes, ledit groupe réactif permettant de moduler la juxtaposition desdites sondes de manière à augmenter la probabilité de transfert d'énergie entre lesdits colorants. L'invention concerne en outre un procédé permettant de détecter la présence d'un protéine de fusion dans une cellule au niveau d'une seule cellule.

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