Method and product for regulating cell responsiveness to...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/54 (2006.01) A61K 38/45 (2006.01) C12N 9/12 (2006.01) C12Q 1/48 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2160548

The isolated nucleic acid sequence for MEKK, the MEKK amino acid sequence and protein, antibodies to MEKK, and methods for using such sequences, proteins and antibodies are described. The amino acid sequence of MEKK (MEK Kinase), was elucidated from a cDNA sequence encoding a protein of 672 amino acid residues (73 kilodaltons). When MEKK is expressed, it phosphorylates and activates MEK. Phosphorylation and activation of MEK by MEKK is independent of Raf, a growth factor regulated protein kinase that also phosphorylates MEK Thus, MEKK and Raf converge at MEK in the protein kinase network mediating the activation of MAPKs by hormones, growth factors, and neurotransmitters.

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