Method and reagent for detecting peroxidase or hydrogen...

Details Method and reagent for detecting peroxidase or hydrogen... Method and reagent for detecting peroxidase or hydrogen...

G - Physics

01

N

G01N 24/10 (2006.01) C12Q 1/26 (2006.01) C12Q 1/28 (2006.01) G01R 33/60 (2006.01)

Patent

CA 2181500

Method for detecting peroxidase or hydrogen peroxide with high sensitivity. Both peroxidase and hydrogen peroxide are prepared such that one of them is overabundant to the other. Phenoxy radicals are produced from a p-substituted phenol compound by the action of peroxidase in the presence of hydrogen peroxide. The free radicals are trapped by a hydroxy amine compound, and stable radical species are produced. Electron spin resonances of the stable radical species are measured.

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