Method and reagent for lymphocyte defined typing of human...

Details Method and reagent for lymphocyte defined typing of human... Method and reagent for lymphocyte defined typing of human...

G - Physics

01

N

167/37

G01N 33/53 (2006.01) G01N 33/50 (2006.01)

Patent

CA 1100868

ABSTRACT A cellular method for typing lymphocyte defined human leukocyte antigens by utilizing human blood leukocytes which have been sensitized to proliferatively respond to the second- ary stimulus of the leukocyte antigens to which they have been sensitized. The sensitization is accomplished by incubating, in vitro, in a mixed leukocyte culture, the leukocytes to be sensitized with leukocytes differing by only a single major histocompatibility complex haplotype or a single HLA-D anti- genic determinant.

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