C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/54 (2006.01) C12Q 1/26 (2006.01) C12Q 1/32 (2006.01) C12Q 1/34 (2006.01) C12Q 1/48 (2006.01)
Patent
CA 2358765
The present invention provides a method for determining 1,5-anhydroglucitol (1,5-AG) in a sample containing 1,5-AG, maltose and glucose, which comprises: converting maltose in the sample into glucose using an enzyme system capable of converting maltose into glucose; converting glucose into a compound which is not phosphorylated by 1,5-anhydroglucitol 6-phosphorylating enzyme system (AG-6P-ES) or dehydrogenated by the action of 1,5-anhydroglucitol-6-phosphate dehydrogenase (AG-6PDH), using an enzyme system capable of converting glucose into said compound; converting 1,5-AG into 1,5-anhydroglucitol- 6-phosphate (1,5-AG-6P) using the AG-6P-ES; dehydrogenating the formed 1,5-AG-6P with AG-6PDH in the presence of an oxidized coenzyme; and determining the component formed or reduced by the dehydrogenation reaction. A reagent and a reagent kit useful in this method are also provided.
Miike Akira
Sasaki Mihoko
Tazoe Sakae
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