Method and reagent for the determination of iron

G - Physics – 01 – N

Patent

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G01N 33/52 (2006.01) G01N 1/28 (2006.01) G01N 33/72 (2006.01) G01N 33/84 (2006.01) G01N 33/90 (2006.01) G01N 33/96 (2006.01)

Patent

CA 2140738

In order to determine iron in serum by releasing the bound iron through addition of a protein denaturing agent, reduction of the released iron to Fe2+, addition of a colour reagent solution and photometric measurement of the colour complex that forms, a mixture of a denaturing agent containing urea or a urea derivative and a fatty alcohol polyglycol ether are added to the sample solution.

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