Method and reagent for the differentiated determination of...

Details Method and reagent for the differentiated determination of... Method and reagent for the differentiated determination of...

G - Physics

01

N

167/45, 195/1.11

G01N 33/573 (2006.01) G01N 33/577 (2006.01)

Patent

CA 1293209

Abstract The subject of the invention is a method and a reagent for the differentiation of the liver isoenzyme and bone isoenzyme of alkaline phosphatase and selective determination of the differentiated isoenzymes by measurement of the alkaline phosphatase activity, the differentiation of the two isoenzymes being performed by means of a monoclonal antibody which is directed against one of the two isoenzymes and has only a slight cross-reactivity with the other isoenzyme. The application furthermore relates to the monoclonal antibody suitable for the differentiation of the two isoenzymes. These are obtained by immunization of experimental animals with pre-purified alkaline phosphatase from liver or bone, fusion of B-lymphocytes, cloning and cultivation of the hybridoma cells thus formed, and isolation of the monoclonal antibody from the latter.

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