Method and reagent for the specific determination of mrna

Details Method and reagent for the specific determination of mrna Method and reagent for the specific determination of mrna

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2181417

Method for the determination of mRNA which is essentially characterized in that a hybrid formed in solution is coupled to a heat-stable solid phase; subsequently, a reverse transcriptase and PCR reaction are carried out in one single reaction vessel. It has proven to be particularly advantageous if the oligonucleotide necessary for the hybridization is labeled with biotin, the solid phase is coated with streptavidin or avidin, and the reaction is carried out without RNase. A corresponding kit is also described.

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