Method and reagent of colorimetry employing an...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/32 (2006.01) G01N 21/78 (2006.01) G01N 31/00 (2006.01) G01N 33/50 (2006.01) G01N 33/52 (2006.01) G01N 33/66 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2471660

A method of colorimetry by which reliable analysis can be easily performed in a short time. An aqueous solution containing a bipyridyl/copper complex and glucose dehydrogenase is infiltrated into a filter paper and this paper is dried. When a sample, e.g., blood, is dropped on this paper, the bipyridyl/copper complex assumes a red-brown color depending on the glucose concentration. The color is measured. Since this reaction is a one-stage reaction, it occurs in as short as up to 5 seconds. The found value is highly reliable because this reaction needs neither hydrogen peroxide nor oxygen.

L'invention porte sur un procédé de colorimétrie au moyen duquel il est possible de réaliser facilement et brièvement une analyse fiable. Selon ce procédé, une solution aqueuse contenant un complexe bipyridyl/cuivre et une glucose déshydrogénase est introduite dans un papier filtre qui est ensuite séché. Lorsqu'on fait tomber goutte à goutte un échantillon, tel que du sang, sur ce papier, le complexe bipyridyl/cuivre prend une couleur rouge-brun en fonction de la concentration en glucose. La couleur est ensuite mesurée. Cette réaction s'effectuant en une étape, elle ne prend pas plus de 5 secondes. La valeur trouvée est extrêmement fiable du fait que cette réaction ne nécessite ni peroxyde d'hydrogène, ni oxygène.

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