C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01)
Patent
CA 2468854
The present invention concerns a system for isolating, depleting, or separating a targeted nucleic acid, such as rRNA, from a sample comprising targeted and nontargeted nucleic acids. It effects a way of enriching for nontargeted nucleic acids, such as mRNAs. The invention further concerns methods of implementing the system and kits for implementing the system, which involves at least one bridging nucleic acid comprising 1) a targeting region complementary to a region on the targeted nucleic acid and 2) a bridging region complementary to the capture region of a capture nucleic acid that comprises a nonreactant structure. The nonreactant structure can be used to isolate the hybridizing molecules after incubation under conditions that allows hybridization.
L'invention porte sur un système d'isolement, appauvrissement ou séparation d'un acide nucléique ciblé tel qu'un ARNr d'avec un échantillon comprenant des acides nucléiques ciblés ou non ciblés, et consistant en un moyen d'enrichissement en acides nucléiques non ciblés tels que des ARNm. L'invention porte également sur des procédés et des trousses de mise en oeuvre du système, qui comprennent au moins un acide nucléique passerelle comportant: 1) une région à cibler complémentaire de l'acide nucléique ciblé, et 2) une région passerelle complémentaire de la région d'un acide nucléique de capture à structure non réactive, ladite structure pouvant servir à isoler les molécules hybridantes après incubation dans des conditions permettant l'hybridation.
Murphy George L.
Whitley J. Penn
Ambion Inc.
Kirby Eades Gale Baker
LandOfFree
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