Method and system for enhanced production of commercially...

Details Method and system for enhanced production of commercially... Method and system for enhanced production of commercially...

Category

C - Chemistry, Metallurgy

Subcategory

12

Subsubcategory

N

IPC codes

C12N 15/75 (2006.01) C12N 1/21 (2006.01) C12N 15/11 (2006.01) C12N 15/31 (2006.01) C12N 15/62 (2006.01) C12N 15/67 (2006.01) C12Q 1/68 (2006.01) G01N 33/566 (2006.01)

Type

Patent

Patent number

CA 2156425

Description

The invention provides a method and expression system for enhancing secretion of hyperproduced homologous and heterologous exoproteins in gram-positive bacteria such as Bacillus sp. The method and system comprise overproduction of PrsA protein in a grampositive bacterial host capable of also overproducing at least one exoprotein of interest. Use of the method and system of the invention results in greatly enhanced secretion of the synthesized exoproteins into the growth medium. Once in the growth medium these secreted exoproteins can be recovered and purified in a straightforward manner.

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