Method and system for multi-stage isoelectric focussing

G - Physics – 01 – N

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G01N 27/447 (2006.01)

Patent

CA 2489077

The invention relates to a method of separating at least two biocomponents contained in a liquid including having different pI values. The method comprising the steps of: i. providing a first separating path with at least one separating layer comprising one or more pH active components with pH active groups, ii. applying the liquid with the biocomponents to the separating coating, iii. applying a voltage over the separating path, iv. allowing at least some of the biocomponents to travel towards one of the electrodes to one or more collection stations, v. collecting the once separated biocomponents from at least one collection station. The invention also relates to a separating system for use in the method. The separation system comprises a set of separating paths, the separating system comprising 2 or more separating paths that differ from each other with respect to the pH value of the separating coating.

Cette invention a trait à un procédé permettant de séparer dans un liquide au moins deux composants biologiques ayant différentes valeurs de points isoélectriques (pHi). Le procédé consiste, (i), à former un premier trajet de séparation avec au moins une couche de séparation comprenant un ou plusieurs composants à pH actif possédant des groupes à pH actif, (ii), à déposer le liquide renfermant les composants biologiques sur l'enduit de séparation, (iii), à appliquer une certaine tension sur le trajet de séparation, (iv), à permettre le déplacement d'au moins quelques composants biologiques vers une des électrodes en direction d'une ou de plusieurs stations de collecte et (v), à recueillir les composants biologiques dans au moins une station de collecte. L'invention porte également sur un système de séparation, utilisable dans le cadre de ce procédé. Ce système, qui comporte un ensemble de trajets de séparation, comporte deux ou plusieurs trajets de séparation différant relativement au pH de l'enduit de séparation.

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